United States securities and exchange commission logo August 18, 2020 Sandra Gardiner Chief Financial Officer Pulse Biosciences, Inc. 3957 Point Eden Way Hayward, California 94545 Re: Pulse Biosciences, Inc. Registration Statement on Form S-3 Filed August 14, 2020 File No. 333-246346 Dear Ms. Gardiner: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Laura Crotty at (202) 551-7614 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Andrew Hoffman